A Comparative Study on the Effect of Coconut Oil and Sesame Oil on Cutaneous Wound Healing in Albino Rats Using Routine (H&E) and Special Histological Stains

Abstract

The failure of wounds to heal naturally is one of the major challenges facing specialists in this field, prompting a search for safe and effective treatments to accelerate wound healing and improve patients' quality of life. Amidst growing interest in natural therapies and alternative medicine, plant oils have emerged as a promising therapeutic option for accelerating wound healing, owing to their antioxidant, anti-inflammatory, and antimicrobial properties. The experiment was designed according to Valizadeh's method with some modifications. The experimental animals were randomly divided into three groups of 12 white mice each (a control group without treatment, a group treated with coconut oil, and a group treated with sesame oil). The mice were of similar weights and were divided into three groups at three intervals (10, 20, and 30 days) after the start of treatment.

The results of the study showed that sesame oil and coconut oil contributed to accelerating wound healing in white mice compared to the control group by improving tissue regeneration and reducing the inflammatory response. Histological examinations using routine and special stains also showed improved collagen fiber organization, granulation tissue formation, and epidermal remodeling. The healing efficiency of the two oils differed depending on their active components. Histological evaluation confirmed the importance of using routine and special stains to monitor the stages of wound healing and accurately assess histological changes.